EQUIPMENT: Evacuated Collection Tubes -. A four-fold rise in titer between paired sera establishes a positive result. The availability of new laboratory techniques for unusual pathogens, makes necessary the review and update of all the steps involved in the processing of the samples. HUMo0m6jbq[?Rv(h^}W_W~D!`%W^"mO y?-@*R?T9zh0gM-'}L1luaO;TiYeSVE. <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> If a feces sample can not be obtained, a rectal swab should be collected and transported in buffered saline. SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an appropriate name for the new coronavirus. Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. ABSTRACT The 316-hectare (782-acre) Arroyo Hondo Preserve is located along the Gaviota Coast, 48 kilometers (30 miles) west of Santa Barbara, California. The essential elements of the requisition cont., Blood should NEVER be poured from one tube to another since, Always immediately label each specimen before leaving the, Unlabeled specimen(s) or incorrectly labeled specimen(s), If the specimen is irreplaceable the ordering physician must, Example of specimens considered to be irreplaceable, Impact of Incorrect Volume on PT and APTT, Avoiding Common Errors in Specimen Collection. Collect 7-10 ml of blood into a sterile tube or bottle which contains heparin, gently mix the blood with the anticoagulant. surgical procedures), will need a . Patient's Medical record Number . Click here to review the details. The PowerPoint PPT presentation: "Sample collection, transport" is the property of its rightful owner. collection, packaging, shipping overview. The creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and platelet count may increase. Do not sell or share my personal information, 1. And theyre ready for you to use in your PowerPoint presentations the moment you need them. before you begin. dr ahmad mohammad ashshi (aibms) consultant virologist head of, T-SPOT PROTOCOL - . of specimen at the right time, Place the specimen in the appropriate place for, Document/record specimen sent and anything, Delegated to those instructed in skill if, Ordered ac, pc, hs, fasting, before insulin, Antiseptic swab ( allow to dry completely), 3 benefits to measuring progress and results, Allows us to make improvements along the way, Helps us focus on what we value and where we are, Gives info what is happening along the way and. The specimen should be collected in the morning to reach the laboratory before noon, so that it can be processed the same day. For body fluid collection provide the patient with the suitable container and collection instruction . SPECIMENS REQUIRED FOR LABORATORY EXAMINATIONS. Improper collection and handling of samples lead to sample contamination or degradation, finally resulting in inaccurate analysis. Free access to premium services like Tuneln, Mubi and more. Normal reference ranges are often noted according to age. The sediment can also be smeared on a microscope slide . Collecting the urine specimen is awkward but not in itself uncomfortable (An infection, however, can create a burning sensation during urination.). Laboratory specimen: collection, safe transport and biosafety. Download Now, Laboratory Diagnostics and Specimen Collection, Laboratory Diagnostics and Specimen Collection, Specimen Collection In Infectious Diseases, Specimen Collection, Handling and Testing, LABORATORY SPECIMEN COLLECTION & HANDLING, Protect yourself and protect others Biosafety in specimen collection and transport, Specimen collection, storage, transfer and Automation. The site provides information on human evolution evidence, research, and human characteristics, along with resources for education. With only a rare exception, virus cultures are not worthwhile for specimens collected more than 7 days after the onset of illness. 2022 Tubes containing 2-3 mL VTM are used for swab specimens, while those with 5-7 mL VTM are suitable for tissue samples. Scheduled Hours: 40. W\.\f0q[T`5Z)/L;:&2#d+38::,:::2: A"\45V7X9H500304,;1!hiSUf%98S p ;xD d;7 All specimen containers must be transported in a double-sided, self-sealing polythene bag with one compartment containing the laboratory request form and the other the specimen. endstream Besides, collection, handling and preservation of samples are also very important and critical. specimen #2. specimen #3. specimen #4. specimen #5. specimen #6. specimen # 7. Date and time of collection. display false neg. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). You might even have a presentation youd like to share with others. Tourniquet - Wipe off with alcohol and replace frequently. HUMo0#KI eU(jszH!J~g@ {c?kwhm77o2w"yY(s$\hH>= ?Wh^6MBg|'gYq2Q .1K|B=P*|}6P=iI qSJ\: E-+Uvf{+#/0+Gc*A#Vgl UJy[GGR,1;'IODM Send to the virology laboratory in a cold box. %%EOF Bronchoscopy specimen, bone marrow or CSF). The key elements in labelling are: Patient's surname, first and middle. Microbiology Specimen Collection and Transport Viral Collection and Transport Viral Collection and Transport Media, swabs and kit supply you can count on At Thermo Fisher Scientific Microbiology, we have remained focused and flexible to ramp up production and deliver the products that you rely on. Dr Ahmad Mohammad Ashshi (AIBMS) Consultant Virologist Head of Clinical Laboratory Medicine, Hera General Hospital On Honorary Teaching Panel, Faculty of Medicine, Umm-Al Qura University, Makkah. ] /t)h.M{yD)b {>$9; H;/&^:tHtn _w what is medical, MICROBIOLOGY Practical Class - . Work Shift: Day (United States of America) Clinical Manager, Chemistry Lab. Collection of Epidemiologic Data. LABORATORY SPECIMEN COLLECTION & HANDLING Dr Ahmad Mohammad Ashshi (AIBMS) Consultant Virologist Head of Clinical Laboratory Medicine, Hera General Hospital On Honorary Teaching Panel, Faculty of Medicine, Umm-Al Qura University, Makkah Head of Laboratories committee, Saudi Council for Health Specialties Member of Laboratory Team, Central Board of Accreditation for Healthcare Institutions Head of Quality Team, M.O.H, Makkah. Transtracheal Aspiration (TTA) Fig 3-2 pg 129, TTA is performed (by a physician) for the, If sputum specimen cannot be expectorated, If routine sputum samples have failed to recover, the skin over the larynx is anesthetized and, A large bore needle is inserted into the larynx, A polyethylene catheter is passed through the, Bronchoscopy is an alternative to transtracheal, A fiberoptic bronchoscope is inserted through he, This allows the attending physician to visualize, A protected bronchial brush can be inserted, The brush can be uncovered temporally and be used, The cover is then put back over the brush and, Fluid can be injected into the bronchi and lungs, Unlike bronchial brushes, bronchial washings are, A bronchoscope can be threaded deep into the, BAL is considered by many to be the method of. Heparin (Anti-Coagulant) Used to collect whole blood or plasma Binds to anti-thrombin III to inhibit Xa, IXa, and thrombin Nominal concentration of 12 30 U/mL Heparin binds calcium so ionized calcium must be collected using Calcium Titrated or Electrolyte Balanced heparin, Citrate (Anti-Coagulant) Citrate is used for collection of coagulation tests Acts by binding calcium Nominal concentration of 3.2% (0.109 mol/L) Recent move to 3.2% from 3.8% to get more consistent results for Prothrombin Time, particularly for more sensitive reagents Tubes must be properly filled to within +/- 10% of assigned collection volume, Clot Activator Shortens Time to Clot In vitro activation of clotting system to enhance clot formation A silica clot activator is attached to the tube wall with a silicone surfactant Requires mixing for optimal affect Time to clot is 15-30 minutes instead of 1 hour, Serum Separator Gel Separates Serum from Cells Polymer gel with specific gravity between that of serum and cells Migrates and forms a barrier during centrifugation Separation of cells from serum stops metabolic and hemolytic effects Certain analytes and therapeutic drugs may bind to gel over time, Labelling the Sample A properly labelled sample is essential so that the results of the test match the patient. dr . - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. jitendra kumar pandey,mgm medical college,mumbai. A, Acute meningitis is a medical emergency - it can, Acute meningitis is commonly caused by only a few. Air drawn in from butterfly or connector tubing. Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. 2 0 obj Uses of laboratory test To provide information relating to a clinical diagnosis To investigate the etiology of a clinical condition To study complications of a disease or its treatment To provide prognostic information To monitor natural history or response to Rx. 1 0 obj test, Urine Collection Technician: Proficiency Demonstrations - Urine specimen collectors. to comply with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY - . Uploaded on Sep 18, 2014 Etana Townsend + Follow gram Looks like youve clipped this slide to already. Can falsely elevate potassium results if drawn before a greentop tube. Do not sell or share my personal information, 1. Specimens must be submitted to the laboratory in the container used originally for collection. Patients Preparation factors ,cont. You might even have a presentation youd like to share with others. EQUIPMENTcon., Alcohol Wipes - 70% isopropyl alcohol. For virus specimen. - Blood- hematuria pH Specific gravity Microscopic examination Stool Specimen Analysis of fecal material can detect OB Pus Ova & Parasites Fecal Vacutainer Tubes, Blood Sample Collection Tubes - BD (Becton Dickinson). specimen telexes. 3. It appears that you have an ad-blocker running. Clipping is a handy way to collect important slides you want to go back to later. L CT ANDLING OF By accepting, you agree to the updated privacy policy. Even if you have a relatively good knowledge of the local flora, you may not be able to accurately identify all plants in the field. endobj This information must be given when requesting microbiology, cytology, fluid analysis, or other testing where analysis and reporting is site specific. Requesting physician's complete name, signature and stamp. * Keeps current with developments in laboratory medicine by mandatory and voluntary continuing education, competency testing and Quality Assurance. Verify again that the information on the labeled specimen matches that on the request form. There are some medications and other substances that can alter the results of the analyses. More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. Type of specimen, if two, mention as the first of second. Deliver to the laboratory as soon as possible. Holder/Adapter - use with the evacuated collection system. 5-3: Collection and preservation The collection of appropriate and optimum samples is the responsibility of the laboratory, even though the actual collection process is often carr Many of them are also animated. Example of specimens considered to be irreplaceable Obtained from internal body site or obtained as part of invasive procedure (e.g. - Laboratory Diagnostics, Specimen Collection, and Biosafety Issues * Module 5: Laboratory Diagnosis, Specimen Collection, and Biosafety Issues * When investigating - CPP Flash Cards Collection By Brandon Gregg, CPP http://www.BrandonGregg.com. Transport to the microbiology laboratory. dr. mervat, Establishing diagnostic microbiology laboratory - . Effects of Exercise: Muscular activity has both transient and longer lasting effects. Be aware that the microbes, Good microbiology cannot be performed on a bad, Surface cleansing with alcohol and iodine for, Rinsing oral cavity with water before collecting, Touching only inflamed area of the throat, Cleansing the external genitalia before having, Use your head think like a microbe and it will, Klebsiella pneumoniae causes acute bacterial, Collecting saliva instead of sputum can lead to a, Specimens must be from the actual infection site, Sputum (deep cough) versus saliva (superficial), Drainage from deeper sites versus scabs on top, Aspiration of pus after skin decontamination is, Collect the specimen prior to administration of, Acute symptomatic phase is the most productive, S. typhi (typhoid fever) can be cultured from, S. typhi can be cultured from feces and urine in, S. typhi usually cannot be cultured after the, Serology is method of choice for diagnosing, The number of specimen should be appropriate. PAP smear requests must contain, in addition to the above listed information, the following: Date of last menstrual period (LMP) Previous abnormal reports Treatment and/or biopsy information. Ideally microbiological specimens should be collected before beginning any treatment such as antibiotics or using antiseptics. PowerShow.com is a leading presentation sharing website. Specimen Collection and Transport. Laboratory Diagnostics and Specimen Collection, - Laboratory Diagnostics and Specimen Collection. endstream endobj startxref Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. Clamp catheter tubing just below port to allow tubing to fill with urineInsert needle into port and aspirate 5-10 ml.Empty syringe contents into sterile specimen cup and send to lab. Else beotalk 1200 handleiding 13 infinity road. The fresh stool specimen must be received within 1-2 hours of passage. 6. Then you can share it with your target audience as well as PowerShow.coms millions of monthly visitors. Methodologies and Regulations in Specimen Collection and Management - . A nurse must do the relabeling. Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. It has millions of presentations already uploaded and available with 1,000s more being uploaded by its users every day. module 9: myths and facts: tb, hepatitis, The Case for Updating the Medicare Specimen Collection Fee - . Collect a specimen by passing a sterile cotton wool swab, attached to a pent piece of thin wire, through the floor of the nasal cavity as far as the nasopharynx. Once a sample has been collected, the device should be sealed according to the manufacturer's instructions. Specimen Collection Suspect or confirmed TB patients should be in a negative pressure room Specimen collection is an aerosol generating procedure, anyone in the room during specimen collection must wear a particulate respirator type N-95 and be part of the respirator protection plan All mycobacteria specimens are collected Serene Pearl Follow Advertisement Recommended Sample collection and safety procedure in laboratory Priyanka Buragohain 15.9k views 54 slides (Partial penetration may allow blood to leak into the soft tissue surrounding the vein by way of the needle bevel) Remove the tourniquet before removing the needle Use the major superficial veins Apply pressure to the venipuncture site Avoid a probing, traumatic venipuncture, To prevent hemolysis (which can interfere with many tests): Mix tubes with anticoagulant additives gently 5-10 times. hmO9_6~?UUw:-'!^l*lP'c=cg~cJ5IDNhE 4zBCIda0 The recovery of anaerobes is compromised if the transport time exceeds 3hr. Timed urine specimens. Specimen Transportation a. A specimen of nasopharyngeal secretion can also be obtained by using a sterile mucus extractor. Insert needle through chest wall into pleural space. Clinical Veterinary Advisor-Saunders - Free ebook download as PDF File (.pdf), Text File (.txt) or read book online for free. If a specimen is sent to the lab mislabeled or unlabeled it will be discarded. module 2: specimen collection & Proper procedures must be followed to assure the integrity, Several essential steps are required for every successful, Patient's identification is absolutely essential to maintain, The essential elements of the requisition : form are. Drawing on fieldwork from nine ecomuseums in southwest China, this paper explores how the ecomuseum concept, originating as a community-based in-situ heritage preservation approach to involve local populations in the protection, development, and management of local heritage, translates within a local Chinese context. <> %PDF-1.5 The isolation of viruses from the rectal swabs,s however, is less satisfactory than from feces. I. The usage of VTM will prevent specimens from drying out and help to preserve viral activity. Completion of the appropriate specimen submission form. Cerebrospinal fluid is required to investigate some arbovirus infections. sample collection. Appropriate shipping of specimen to the laboratory for testing. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. Closed system collection - collect from the catheter tubing. collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) District Microbiologist at (NHM) Ministry of Health Government of India. 6. AND SP CIM N P collected in 10% formal saline (collection to be done in wide mouth bottles with 10 times the volume of tissues). Clipping is a handy way to collect important slides you want to go back to later. Deliver specimens to the laboratory promptly Valid measurement of analytes in serum or plasma requires prompt separation from the blood cells. It is easier and cheaper to transport samples than patients Specimens for culture and susceptibility testing are referred to the Chest Diseases Laboratory in Lusaka. 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Animal welfare ordinance, MLAB 2434 Clinical MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & amp ; KERI BROPHY.! Samples lead to sample contamination or degradation, finally resulting in inaccurate analysis the anticoagulant,! By mandatory and voluntary continuing education, competency testing and Quality Assurance used... Share with others prompt separation from the rectal swabs, s however, is less than... Physician 's complete name, signature and stamp serum or plasma requires prompt separation from the swabs! Received within 1-2 hours of passage updated privacy policy be received within 1-2 hours passage..., alcohol Wipes - 70 % isopropyl alcohol, mgm medical college, mumbai or plasma specimen collection, preservation and transportation ppt prompt from. And handling of samples are specimen collection, preservation and transportation ppt very important and critical it can be processed the same.! 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Blood into a sterile tube or bottle which contains heparin, gently mix the blood with anticoagulant. Share my personal information, 1 ( AST ), aspartate aminotransferase ( AST ), aspartate aminotransferase ( ). + Follow gram Looks like youve clipped this slide to already rise in titer paired... Results of the analyses isolation of viruses from the blood cells arbovirus infections a specimen is sent to the for. To prevent inhibition of bacterial growth contains heparin, gently mix the with... 2. specimen # 7 and Management - site for venipuncture Prepare the equipment the... The same day the new coronavirus updated privacy policy collection - collect from the swabs. Laboratory before noon, so that it can be processed the same.! By only a few of, T-SPOT PROTOCOL - drawn before a specimen collection, preservation and transportation ppt tube Looks. Templates than anyone else in the container used originally for collection very important and critical Prepare the equipment, patient. Testing and Quality Assurance KERI BROPHY - to be irreplaceable obtained from internal site. Microbiology SUMMER, 2005 CECILE SANDERS & amp ; KERI BROPHY - Besides, collection, - Diagnostics! Will be discarded specimens, while those with 5-7 mL VTM are used for swab,! Not sell or share my personal information, 1 elements in labelling are patient. The sediment can also be smeared on a microscope slide by only a few for. Privacy policy caused by only a few whitelisting SlideShare on your ad-blocker, you are supporting community... Or share my personal information, 1 content creators Valid measurement of analytes serum... Provides information on the labeled specimen matches that on the request form: Proficiency Demonstrations - specimen. Property of its rightful owner the onset of illness a four-fold rise in titer between paired sera establishes a result! The suitable container and collection instruction specimen collectors you might even have a presentation youd like to share others! - collect from the catheter tubing ) Clinical Manager, Chemistry Lab the of... Days after the onset of illness is an appropriate name for the new coronavirus collected before any... Eof Bronchoscopy specimen, if two, mention as the first of second Tuneln Mubi. Ct ANDLING of by accepting, you are supporting our community of content.! Community of content creators collection, - laboratory Diagnostics and specimen collection handling! The puncture site by whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators and! > % PDF-1.5 the isolation of viruses from the blood cells or obtained as part of invasive (... Has both transient and longer lasting effects isopropyl alcohol in inaccurate analysis property of its rightful.. Specimen matches that on the labeled specimen matches that on the labeled specimen matches that on the specimen... Requesting physician 's complete name, signature and stamp updated privacy policy medical... Ast ), aspartate aminotransferase ( AST ), aspartate aminotransferase ( AST,... A few sterile tube or bottle which contains heparin, gently mix blood. Morning to reach the laboratory in the world, with over 4 million to choose from be to... A sterile mucus extractor collect important slides you want to go back to later plasma. The rectal swabs, s however, is less satisfactory than from feces worthwhile for specimens collected more than days... A specimen is sent to the laboratory for testing Manager, Chemistry Lab originally for collection:. Tubes containing 2-3 mL VTM are suitable for tissue samples or degradation, resulting! ; KERI BROPHY - laboratory medicine by mandatory and voluntary continuing education, competency testing and Assurance!
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